Beilstein J. Nanotechnol.2015,6, 517–528, doi:10.3762/bjnano.6.54
. Thus, a prior interaction of NPs with pulmonarysurfactant components will occur. In the present study we explored the impact of pulmonarysurfactant on the cytotoxic potential of amorphous silica nanoparticles (aSNPs) using in vitro mono- and complex coculture models of the air–blood barrier
culture period.
Keywords: air–blood barrier; cytotoxicity; inflammatory response; pulmonarysurfactant; silica nanoparticles; Introduction
Biological barriers of the human body which directly interface the external environment have, besides their actual physiological function, the vital task of
protective alveolar surfactant lining layer (10–20 nm in thickness), that covers the entire alveolar surface [2]. It has already been shown that regardless of the NP surface properties they will be submerged in the aqueous phase of the alveolar lining layer after crossing the pulmonarysurfactant layer [3][4
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Figure 1:
Comparison of cytotoxic effects on A549 after 4 h stimulation with aSNPs displaying different surfa...
Beilstein J. Nanotechnol.2013,4, 933–940, doi:10.3762/bjnano.4.105
alveolar region [10][11][12]. Once deposited there, nanoparticles are found to interact with the epithelial lining fluid including pulmonarysurfactant, lung macrophages and epithelial cells [13][14][15]. Depending on their physico-chemical properties, a small portion of the inhaled nanomaterials may even
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Figure 1:
Representative transmission (A) and scanning electron (B) images of PVP-coated AgNP.